All lanes : Anti-PSMC5 antibody [EPR13565(B)] (ab178681) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : HepG2 cell lysateLane 3 : A431 cell lysateLane 4 : 293T cell lysateLysates/proteins at 10 µg per lane.
Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labeling PSMC5 with ab178681 at 1/50 dilution.
Immunofluorescent analysis of HeLa cells labeling PSMC5 with ab178681 at 1/100 dilution.
Flow cytometric analysis of permeabilized Hela cells labeling PSMC5 with ab178681 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).
Western blot analysis on immunoprecipitation pellet from (Lane 1) 293T cell lysate or (Lane 2) 1XPBS (negative control) using ab178681 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.