All lanes : Anti-PSMC5 antibody [EPR13566(B)] - C-terminal (ab180495) at 1/10000 dilutionLane 1 : HepG2 lysateLane 2 : HeLa lysateLane 3 : 293T lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PSMC5 with ab180495 at 1/250 dilution followed by ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG secondary and Counter stained with Hematoxylin.
Flow Cytometric analysis of HeLa cells (2% paraformaldehyde-fixed) labeling PSMC5 with ab180495 at 1/30 dilution (green) compared to a negative control Rabbit monoclonal IgG (red) follwed by Goat anti rabbit IgG (FITC) secondary at 1/75 dilution.
Immunofluorescent analysis of 293T cells (4% paraformaldehyde-fixed) labeling PSMC5 with ab180495 at 1/250 dilution (red) followed by with Goat anti rabbit IgG (Dylight 555) . DAPI staining (blue).