Flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling PSMD9 with ab181998 at 1/300 dilution (red) or a rabbit IgG (negative) (green), followed by secondary Goat anti-rabbit IgG (FITC) at 1/150 dilution.
Immunofluorescent analysis of HeLa cells (-20°C acetone-fixed) labeling PSMD9 with ab181998 at 1/500 dilution followed by Goat anti rabbit IgG (Dylight 555) secondary and counter-stained with DAPI (blue).
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PSMD9 with ab181998 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Immunofluorescent analysis of HepG2 cells (paraformaldehyde-fixed, 4%) labeling PSMD9 with ab181998 at 1/500 dilution. A Goat anti rabbit IgG(Alexa Fluor® 555) was used as secondary antibody. Counterstained with DAPI (blue).
All lanes : Anti-PSMD9 antibody [EPR12947] (ab181998) at 1/1000 dilutionLane 1 : Human fetal liver lysateLane 2 : HeLa cell lysateLane 3 : HepG2 cell lysateLane 4 : 293 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L) -HRP at 1/1000 dilution