All lanes : Anti-PSME2 antibody [EPR14931] (ab183727) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : PC3 cell lysateLane 3 : HepG2 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human kidney clear cell carcinoma tissue labeling PSME2 with ab183727 at 1/100 dilution, followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling PSME2 with ab183727 at 1/100 dilution, followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Immunofluorescent analysis of HeLa cells (-20°C acetone-fixed) labeling PSME2 with ab183727 at 1/500 dilution, followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Immunofluorescent analysis of HepG2 cells (paraformaldehyde-fixed, 4%) labeling PSME2 with ab183727 at 1/500 dilution, followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling PSME2 with ab183727 at 1/210 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.