Immunoprecipitation analysis of HepG2 cells labeling PTDSS2 using ab183504 at 1/50 dilution. A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody.
Flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling PTDSS2 with ab183504 at 1/100 followed by Goat anti rabbit IgG (FITC) at 1/150 (pink). Rabbit monoclonal IgG was used as isotype control (green).
Anti-PTDSS2 antibody [EPR14663] - C-terminal (ab183504) at 1/10000 dilution + Hela cell line lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
All lanes : Anti-PTDSS2 antibody [EPR14663] - C-terminal (ab183504) at 1/2000 dilutionLane 1 : HepG2 cell line lysateLane 2 : Human fetal brain tissue lysateLane 3 : Human fetal liver tissue lysateLysates/proteins at 20 µg per lane.SecondaryLane 1 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilutionLanes 2 - 3 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated