Anti-PUF60 antibody
| Name | Anti-PUF60 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab22819 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB ELISA |
| Species Reactivities | Human, Mouse, Rat, Pig |
| Antigen | Synthetic peptide: YDQERFDNSDLSA , corresponding to C terminal amino acids 530-542 of Human PUF60 |
| Description | Goat Polyclonal |
| Gene | PUF60 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-PUF60 antibody (ab22819) at 0.1 µg/ml + HEK293 cell lysate (35µg in RIPA buffer)
ICC/IF image of ab22819 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22819, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-PUF60 antibody (ab22819) at 1 µg/ml + A431 lysate (35µg protein in RIPA buffer).