All lanes : Anti-PUF60 antibody [GT677] (ab184538) at 1/5000 dilutionLane 1 : 293T whole cell lysateLane 2 : A431 whole cell lysateLane 3 : HeLa whole cell lysateLane 4 : HepG2 whole cell lysateLysates/proteins at 30 µg per lane.
Anti-PUF60 antibody [GT677] (ab184538) at 1/1000 dilution + Mouse brain lysate at 50 µg
Anti-PUF60 antibody [GT677] (ab184538) at 1/1000 dilution + Rat brain lysate at 50 µg
Immunofluorescent analysis of A431 cells (fixed in 4% paraformaldehyde, RT for 15 mins) labeling PUF60 with ab184538 at 1/500 dilution (Green). Blue: Hoechst 33342 staining.
Immunohistochemical analysis of paraffin-embedded AGS gastric carcinoma xenograft labeling PUF60 with ab184538 at 1/100 dilution.