Anti-PWP1 antibody [EPR16130] (ab190794) at 1/10000 dilution + Jurkat cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-PWP1 antibody [EPR16130] (ab190794) at 1/1000 dilutionLane 1 : Hela cell lysateLane 2 : 293 cell lysateLane 3 : HepG2 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue, labeling PWP1 with ab190794 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.
Flow cytometry analysis of PWP1 expression in Jurkat cells using ab190794 at 1/30 dilution (red) and a rabbit IgG as negative control (green). A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody.