Immunofluorescent analysis of ZR-75-1 cells labeling PYGO1 with ab170607 at 1/10 dilution followed by Alexa Fluor 488-conjugated Goat anti-Rabbit lgG (green). Actin filaments were labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nucleus (blue).
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue labeling PYGO1 with ab170607 at 1/10 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining
Anti-PYGO1 antibody - C-terminal (ab170607) at 1/100 dilution + ZR-75-1 cell lysate at 35 µg