Anti-Pygopus 2 antibody
| Name | Anti-Pygopus 2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab99274 |
| Prices | $384.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IP IHC-P |
| Species Reactivities | Mouse, Human, Rat, Rabbit, Guinea Pig, Bovine, Dog, Pig, Chimpanzee, Monkey, Ape, Hamster, Orangutan, Elephant |
| Antigen | Synthetic peptide corresponding to a region within the N terminal amino acids 1-50 of Human Pygopus 2 (NP_612157 |
| Description | Rabbit Polyclonal |
| Gene | PYGO2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Pygopus 2 with ab99274 at 1/200 (1µg/mg). Detection: DAB.
All lanes : Anti-Pygopus 2 antibody (ab99274) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µgLane 5 : NIH 3T3 whole cell lysate at 50 µgdeveloped using the ECL technique
ab99274, at 0.4 µg/ml, detecting Pygopus 2 in HeLa whole cell lysate by Western blot analysis following Immunoprecipitation (IP). Detection utilized ECL with a 3 second exposure. For immunoprecipitation, ab99274 was used at 3 µg/mg lysate (1 mg of lysate was used for IP and 20% of IP was loaded). Lane 1; IP using ab99274 Lane 2; IP using a rabbit anti-Pygopus 2 antibody which recognizes a downstream epitope. Lane 3; IP using control IgG.