All lanes : Anti-Pyrophosphatase 1 antibody [EPR11282] (ab181126) at 1/10000 dilutionLane 1 : Anti-Pyrophosphatase 1 antibody [EPR11282] (ab181126)Lane 2 : Anti-Pyrophosphatase 1 antibody [EPR11282] (ab181126)Lane 3 : Anti-Pyrophosphatase 1 antibody [EPR11282] (ab181126)Lysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Flow cytometry analysis of HepG2 cells using ab181126 at a 1/290 dilution (red) or a Rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.
Detection of Human Pyrophosphatase 1 by Western Blot of Immunoprecipitate. MOLT4 whole cell lysate loaded, ab181126 used for blotting immunoprecipitated Pyrophosphatase 1 at a 1/60 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary used at a 1/1000 dilution.