Anti-QARS antibody (ab72957) at 1 µg/ml + human liver tissue lysate at 25 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
All lanes : Anti-QARS antibody (ab72957) at 1 µg/mlLane 1 : QARS transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
ab72957, at 10µg/ml, staining QARS in HeLa cells by immunofluorescence.
Cells were lysed hypotonically in 10 mM HEPES pH 7.6, 10 mM KOAc, 1.5 mM Mg(OAc)2, 0.5 mM DTT followed by douncing, followed by salt adjustment to 130 mM KOAc and addition of 1% NP40 for immunoprecipitation.Lane 1: inputLane 2: supernatant, control antibodyLane 3: supernatant, ab72957 at a 1/50 dilution.Lane 4: beads. control antibodyLane 5: beads, ab72957 at a 1/50 dilution.Whole cell lysate prepared from HeLa cells was loaded at 25000000 cells. The immunoprecipitation step was performed using Protein G. For WB ab72957 used at a 1/1000 dilution.See Abreview