All lanes : Anti-QPRT antibody [4B3] (ab119042) at 1/500 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY QPRT cDNALysates/proteins at 5 µg per lane.
All lanes : Anti-QPRT antibody [4B3] (ab119042) at 1/500 dilutionLane 1 : HepG2 cell extractLane 2 : Hela cell extractLane 3 : HT29 cell extractLane 4 : A549 cell extractLane 5 : COS7 cell extractLane 6 : Jurkat cell extractLane 7 : MDCK cell extractLane 8 : PC12 cell extractLane 9 : MCF7 cell extractLysates/proteins at 35 µg per lane.
HEK293T cells transfected with either a QPRT overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained using ab119042 at 1/100 dilution and then analyzed by flow cytometry.