Anti-RAB14 antibody
| Name | Anti-RAB14 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab28639 |
| Prices | $390.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF ELISA IHC-P WB |
| Species Reactivities | Mouse, Human, Rat, Bovine, Dog, Pig, Zebrafish |
| Antigen | Synthetic peptide derived from a region between amino acids 165-214 of human RAB14 |
| Description | Rabbit Polyclonal |
| Gene | RAB14 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-RAB14 antibody (ab28639) at 0.5 µg/ml + Raji cell lysate 10ug.SecondaryHRP conjugated anti-Rabbit IgG
![Human kidney tissue stained with ab28639 at X400 magnigication. The arrows point to epithelial cells of the renal tubule [positive label].](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/19291_ab28639_2.jpg)
Human kidney tissue stained with ab28639 at X400 magnigication. The arrows point to epithelial cells of the renal tubule [positive label].
ICC/IF image of ab28639 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28639, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-RAB14 antibody (ab28639) at 2 µg/mlLane 1 : HepG2 cell lysateLane 2 : HepG2 cell lysate with blocking peptide at 2 Lysates/proteins at 2 µg/ml per lane.
Product References
A role for Rab14 in the endocytic trafficking of GLUT4 in 3T3-L1 adipocytes. - A role for Rab14 in the endocytic trafficking of GLUT4 in 3T3-L1 adipocytes.
Reed SE, Hodgson LR, Song S, May MT, Kelly EE, McCaffrey MW, Mastick CC, Verkade P, Tavare JM. J Cell Sci. 2013 May 1;126(Pt 9):1931-41.
Proteomic analysis of endocytic vesicles: Rab1a regulates motility of early - Proteomic analysis of endocytic vesicles: Rab1a regulates motility of early
Mukhopadhyay A, Nieves E, Che FY, Wang J, Jin L, Murray JW, Gordon K, Angeletti RH, Wolkoff AW. J Cell Sci. 2011 Mar 1;124(Pt 5):765-75.
Muscle cells engage Rab8A and myosin Vb in insulin-dependent GLUT4 translocation. - Muscle cells engage Rab8A and myosin Vb in insulin-dependent GLUT4 translocation.
Ishikura S, Klip A. Am J Physiol Cell Physiol. 2008 Oct;295(4):C1016-25. doi: