All lanes : Anti-RAB39 antibody (ab99445) at 1 µg/mlLane 1 : Spleen (Mouse) Tissue Lysate Lane 2 : WI38 (Human lung fibroblast cell line) Whole Cell Lysate Lane 3 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.