All lanes : Anti-RAB39B antibody [EPR9651] (ab154826) at 1/100 dilution (unpurified)Lane 1 : Human fetal brain tissue lysateLane 2 : U87-MG cell lysateLysates/proteins at 10 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
All lanes : Anti-RAB39B antibody [EPR9651] (ab154826) at 1/2000 dilution (purified)Lane 1 : Human fetal brain tissue lysateLane 2 : U87-MG cell lysateLysates/proteins at 10 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
All lanes : Anti-RAB39B antibody [EPR9651] (ab154826) at 1/1000 dilution (unpurified)Lane 1 : Fetal brain lysateLane 2 : U87-MG cell lysateLane 3 : SH-SY5Y cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution
Immunocytochemistry/Immunofluorescence analysis of SH-SY5Y cells labelling RAB39B with unpurified ab154826 at 1/10 (red). Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).
Immunocytochemistry/Immunofluorescence analysis of SH-SY5Y cells labelling RAB39B with purified ab154826 at 1/200 (red). Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).
Flow cytometry analysis of U87-MG cells labelling RAB39B with unpurified ab154826 (red) at 1/9. Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.
Flow cytometry analysis of U87-MG cells labelling RAB39B with purified ab154826 (red) at 1/210. Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.