All lanes : Anti-RAB5C antibody [EPR17321] (ab199530) at 1/1000 dilutionLane 1 : HEK293 (Human embryonic kidney) cell lysateLane 2 : HepG2 (Human liver hepatocellular carcinoma) cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-RAB5C antibody [EPR17321] (ab199530) at 1/10000 dilution + U937 (Human histiocytic lymphoma cells) cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-RAB5C antibody [EPR17321] (ab199530) at 1/1000 dilution + Human fetal heart tissue lysate at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling RAB5C with ab199530 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining of Human kidney tissue is observed. Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody and secondary antibody Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.