Anti-Rab9 antibody
| Name | Anti-Rab9 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab45864 |
| Prices | $376.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human Rab9 |
| Description | Rabbit Polyclonal |
| Gene | RAB9A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Rab9 antibody (ab45864) at 1 µg/mlLane 1 : Spleen (Mouse) Tissue Lysate (ab29293)Lane 2 : Spleen (Rat) Tissue Lysate - normal tissue (ab29393)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L (HRP) at 1/3000 dilutionPerformed under reducing conditions.
IHC image of ab45864 staining in mouse spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45864, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.