![Overlay histogram showing THP1 cells stained with ab150011 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab150011, 0.1µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was Mouse IgG1 [MOPC-21] (FITC) (ab18435, 0.1µg/1x106 cells) for 30 min at 22°C. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/19744_Rab9-Primary-antibodies-ab150011-1.jpg)
Overlay histogram showing THP1 cells stained with ab150011 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab150011, 0.1µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was Mouse IgG1 [MOPC-21] (FITC) (ab18435, 0.1µg/1x106 cells) for 30 min at 22°C. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.