Anti-RACGAP1 antibody
| Name | Anti-RACGAP1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab61192 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB ICC/IF ICC/IF ELISA |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic non-phosphopeptide (Human) from around the phosphorylation site of serine 387 (RISPGC) |
| Description | Rabbit Polyclonal |
| Gene | RACGAP1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-RACGAP1 antibody (ab61192) at 1/500 dilutionLane 1 : Cos7 cell extractLane 2 : Cos7 cell extract with peptide
Immunofluorescence analysis of HeLa cells labelled with ab61192 at 1/50 dilution. Samples were treated -/+ peptide (left and right images respectively).
IHC image of ab61192 staining in human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab61192, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.