All lanes : Anti-RAD52 antibody (ab103067) at 1/250 dilution (Milk Blocking - 3%)Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 4 : HeLa (Human epithelial carcinoma cell line) Nuclear LysateLane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 6 : U2OS (Human osteosarcoma cell line) Whole Cell LysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ab103067 (1/200) staining RAD52 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X-100/ PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.See Abreview