Anti-RAD52 antibody [5H9]
| Name | Anti-RAD52 antibody [5H9] |
|---|---|
| Supplier | Abcam |
| Catalog | ab12447 |
| Prices | $379.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | 5H9 |
| Applications | ICC/IF ICC/IF FC WB |
| Species Reactivities | Human |
| Antigen | Human Rad52 recombinant protein expressed in E |
| Description | Mouse Monoclonal |
| Gene | RAD52 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Overlay histogram showing HeLa cells stained with ab12447 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12447,1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG cocktail (1µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
The chromatin remodeler p400 ATPase facilitates Rad51-mediated repair of DNA - The chromatin remodeler p400 ATPase facilitates Rad51-mediated repair of DNA
Courilleau C, Chailleux C, Jauneau A, Grimal F, Briois S, Boutet-Robinet E, Boudsocq F, Trouche D, Canitrot Y. J Cell Biol. 2012 Dec 24;199(7):1067-81.
Preferential localization of hyperphosphorylated replication protein A to - Preferential localization of hyperphosphorylated replication protein A to
Wu X, Yang Z, Liu Y, Zou Y. Biochem J. 2005 Nov 1;391(Pt 3):473-80.