Anti-RAD54 antibody [Rad54 4E3/1]
| Name | Anti-RAD54 antibody [Rad54 4E3/1] |
|---|---|
| Supplier | Abcam |
| Catalog | ab11055 |
| Prices | $392.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | Rad54 4E3/1 |
| Applications | IP WB ICC/IF ICC/IF FC |
| Species Reactivities | Human |
| Antigen | Full length protein (Human) |
| Description | Mouse Monoclonal |
| Gene | RAD54L |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-RAD54 antibody [Rad54 4E3/1] (ab11055) at 1/1000 dilution (in blocker + PBS + 0.1% Tween for 4 hours at 4°C) + HCT116p53wt cells (whole cell lysate) at 30 µgSecondaryAn Alexa Fluor®680 goat anti-mouse polyclonal at 1/10000 dilutionPerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/20157_RAD54-Primary-antibodies-ab11055-7.jpg)
Anti-RAD54 antibody [Rad54 4E3/1] (ab11055) at 1/1000 dilution (in blocker + PBS + 0.1% Tween for 4 hours at 4°C) + HCT116p53wt cells (whole cell lysate) at 30 µgSecondaryAn Alexa Fluor®680 goat anti-mouse polyclonal at 1/10000 dilutionPerformed under reducing conditions.
![Overlay histogram showing HeLA cells stained with ab11055 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11055, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/20158_RAD54-Primary-antibodies-ab11055-10.jpg)
Overlay histogram showing HeLA cells stained with ab11055 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11055, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
PARP3 affects the relative contribution of homologous recombination and - PARP3 affects the relative contribution of homologous recombination and
Beck C, Boehler C, Guirouilh Barbat J, Bonnet ME, Illuzzi G, Ronde P, Gauthier LR, Magroun N, Rajendran A, Lopez BS, Scully R, Boussin FD, Schreiber V, Dantzer F. Nucleic Acids Res. 2014 May;42(9):5616-32.
The RAD51-stimulatory compound RS-1 can exploit the RAD51 overexpression that - The RAD51-stimulatory compound RS-1 can exploit the RAD51 overexpression that
Mason JM, Logan HL, Budke B, Wu M, Pawlowski M, Weichselbaum RR, Kozikowski AP, Bishop DK, Connell PP. Cancer Res. 2014 Jul 1;74(13):3546-55.
Contributions of nucleotide excision repair, DNA polymerase eta, and homologous - Contributions of nucleotide excision repair, DNA polymerase eta, and homologous
Muylaert I, Elias P. J Biol Chem. 2010 Apr 30;285(18):13761-8.
Rad54B targeting to DNA double-strand break repair sites requires complex - Rad54B targeting to DNA double-strand break repair sites requires complex
Murzik U, Hemmerich P, Weidtkamp-Peters S, Ulbricht T, Bussen W, Hentschel J, von Eggeling F, Melle C. Mol Biol Cell. 2008 Jul;19(7):2926-35.