Anti-Rad6 antibody
| Name | Anti-Rad6 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab31917 |
| Prices | $380.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-P WB IHC |
| Species Reactivities | Mouse, Rat, Human, Zebrafish, Rabbit, Chicken, Bovine, Yeast, Xenopus, Drosophila |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Rad6 |
| Blocking Peptide | Human Rad6 peptide |
| Description | Rabbit Polyclonal |
| Gene | UBE2A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Rad6 antibody (ab31917) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear LysateLysates/proteins at 20 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H&L) at 1/15000 dilutionPerformed under reducing conditions.
ab31917 (1/200) stainning Rad6 in RPE-1 cells (green). Cells were fixed in paraformaldehyde, permeabilised with Triton X100 and counterstained with DAPI in order to highlight the nucleus (blue). Please refer to abreview for further details.See Abreview
IHC image of ab31917 staining in human colon carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab31917, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-Rad6 antibody (ab31917) at 1 µg/mlLane 1 : MarkerLane 2 : Zebrafish brain homogenateLane 3 : Zebrafish heart homogenateLane 4 : Zebrafish liver homogenateLane 5 : Zebrafish skeletal muscle homogenateLane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
All lanes : Anti-Rad6 antibody (ab31917) at 1 µg/mlLane 1 : Testis (Mouse) Tissue Lysate Lane 2 : Testis (Rat) Tissue Lysate - normal tissue (ab29388)Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
Melanoma Development and Progression Are Associated with Rad6 Upregulation and - Melanoma Development and Progression Are Associated with Rad6 Upregulation and
Rosner K, Mehregan DR, Kirou E, Abrams J, Kim S, Campbell M, Frieder J, Lawrence K, Haynes B, Shekhar MP. J Skin Cancer. 2014;2014:439205.
Asymmetric nature of two subunits of RAD18, a RING-type ubiquitin ligase E3, in - Asymmetric nature of two subunits of RAD18, a RING-type ubiquitin ligase E3, in
Masuda Y, Suzuki M, Kawai H, Suzuki F, Kamiya K. Nucleic Acids Res. 2012 Feb;40(3):1065-76.
En bloc transfer of polyubiquitin chains to PCNA in vitro is mediated by two - En bloc transfer of polyubiquitin chains to PCNA in vitro is mediated by two
Masuda Y, Suzuki M, Kawai H, Hishiki A, Hashimoto H, Masutani C, Hishida T, Suzuki F, Kamiya K. Nucleic Acids Res. 2012 Nov 1;40(20):10394-407.