Anti-Radixin antibody [EP1862Y]

• Supplier: Abcam
• Catalog: ab52495
• Prices: $99.00, $391.00
• Sizes: 10 µl, 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP1862Y
• Applications: ICC/IF ICC/IF WB IP ICC/IF FC IHC-P
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide corresponding to residues near the N terminus of human Radixin
• Description: Rabbit Monoclonal
• Gene: RDX
• Conjugate: Unconjugated

Product images

Anti-Radixin antibody [EP1862Y] (ab52495) at 1/1000 dilution + HeLa cell lysate at 10 µgSecondaryHRP labeled goat anti-rabbit at 1/2000 dilution

Immunohistochemical staining of paraffin-embedded human ovarian carcinoma using ab52495 at 1/100 dilution.

ICC/IF image of ab52495 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52495, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Overlay histogram showing HeLa cells stained with ab52495 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52495, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Product References

Inversin/Nephrocystin-2 is required for fibroblast polarity and directional cell - Inversin/Nephrocystin-2 is required for fibroblast polarity and directional cell

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Induction of ezrin-radixin-moesin molecules after cryogenic traumatic brain - Induction of ezrin-radixin-moesin molecules after cryogenic traumatic brain

Moon Y, Kim JY, Choi SY, Kim K, Kim H, Sun W. Neuroreport. 2011 Apr 20;22(6):304-8.

miRNA mutations are not a common cause of deafness. - miRNA mutations are not a common cause of deafness.

Hildebrand MS, Witmer PD, Xu S, Newton SS, Kahrizi K, Najmabadi H, Valle D, Smith RJ. Am J Med Genet A. 2010 Mar;152A(3):646-52.

Expression of ezrin radixin moesin proteins in the adult subventricular zone and - Expression of ezrin radixin moesin proteins in the adult subventricular zone and

Persson A, Lindwall C, Curtis MA, Kuhn HG. Neuroscience. 2010 May 5;167(2):312-22.

Participation of ezrin in bacterial uptake by trophoblast giant cells. - Participation of ezrin in bacterial uptake by trophoblast giant cells.

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