Anti-RALA antibody [EPR6468]

• Supplier: Abcam
• Catalog: ab126627
• Prices: $99.00, $313.00
• Sizes: 10 µl, 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR6468
• Applications: WB IHC-P FC
• Species Reactivities: Human
• Antigen: Synthetic peptide, corresponding to a region within Human RALA
• Description: Rabbit Monoclonal
• Gene: RALA
• Conjugate: Unconjugated

Product images

Overlay histogram showing MCF7 cells stained with ab126627 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126627, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

All lanes : Anti-RALA antibody [EPR6468] (ab126627) at 1/1000 dilutionLane 1 : MCF7 cell lysateLane 2 : MOLT4 cell lysateLane 3 : BxPC3 cell lysateLane 4 : HepG2 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP conjugated antibody at 1/2000 dilution

ab126627, at 1/100, staining RALA in formalin fixed, paraffin embedded Human breast carcinoma tissue by Immunohistochemistry.

Product References

Ubiquitously transcribed genes use alternative polyadenylation to achieve - Ubiquitously transcribed genes use alternative polyadenylation to achieve

Lianoglou S, Garg V, Yang JL, Leslie CS, Mayr C. Genes Dev. 2013 Nov 1;27(21):2380-96.