Anti-RALBP1 antibody
| Name | Anti-RALBP1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab56815 |
| Prices | $395.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Applications | WB FC IHC-P |
| Species Reactivities | Human |
| Antigen | Recombinant full length protein, corresponding to amino acids 1-656 of Human RALBP1 |
| Description | Mouse Monoclonal |
| Gene | RALBP1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HEK293 cells stained with ab56815 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56815, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/20435_RIP1-Primary-antibodies-ab56815-1.jpg)
Overlay histogram showing HEK293 cells stained with ab56815 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56815, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
IHC image of ab56815 staining in human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab56815, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Anti-RALBP1 antibody (ab56815) at 1/1000 dilution + HCT116 cell lysate at 30 µgSecondaryHRP-conjugated goat anti-mouse polyclonal IgG at 1/10000 dilutiondeveloped using the ECL techniquePerformed under non-reducing conditions.
Product References
Pax2 regulates a fadd-dependent molecular switch that drives tissue fusion during - Pax2 regulates a fadd-dependent molecular switch that drives tissue fusion during
Viringipurampeer IA, Ferreira T, DeMaria S, Yoon JJ, Shan X, Moosajee M, Gregory-Evans K, Ngai J, Gregory-Evans CY. Hum Mol Genet. 2012 May 15;21(10):2357-69.
Activation and involvement of Ral GTPases in colorectal cancer. - Activation and involvement of Ral GTPases in colorectal cancer.
Martin TD, Samuel JC, Routh ED, Der CJ, Yeh JJ. Cancer Res. 2011 Jan 1;71(1):206-15.