All lanes : Anti-RALBP1 antibody [6C6] (ab175357) at 1/1000 dilutionLane 1 : 293T whole cell lysateLane 2 : A549 whole cell lysateLane 3 : Jurkat whole cell lysateLane 4 : A431 whole cell lysateLane 5 : MCF7 whole cell lysateLane 6 : HeLa whole cell lysateLane 7 : U2OS whole cell lysateLane 8 : K562 whole cell lysateLane 9 : COS7 whole cell lysateLane 10 : 3T3L1 whole cell lysateLane 11 : NRK whole cell lysateLysates/proteins at 25 µg per lane.Secondarygoat anti-mouse-HRP at 1/20000 dilutiondeveloped using the ECL technique
All lanes : Anti-RALBP1 antibody [6C6] (ab175357) at 1/1000 dilutionLane 1 : 293T whole cell lysateLane 2 : A431 whole cell lysateLane 3 : A549 whole cell lysateLane 4 : RALBP1 over-expressing HEK293T cell lysateLysates/proteins at 25 µg per lane.Secondarygoat anti-mouse-HRP at 1/20000 dilutiondeveloped using the ECL technique
Immunoprecipitation of RALBP1 was performed on 293T cells. The antigen:antibody complex was formed by incubating 750 µg whole cell lysate with 2 µg of ab175357 (lane 2). Lane 1 shows lysate only control. For WB detectiion, ab175357 was used at 1/1000 dilution.