Anti-Ran antibody
| Name | Anti-Ran antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab4781 |
| Prices | $385.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ICC/IF ICC/IF IP WB |
| Species Reactivities | Human, Xenopus |
| Antigen | Recombinant full length protein (Human) - His-tagged and purified from E |
| Description | Rabbit Polyclonal |
| Gene | RAN |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Ran antibody (ab4781) at 1/500 dilution + Lysate prepared from human HUH-7 cells at 15000 cellsSecondaryHRP conjugated sheep polyclonal to rabbit IgG at 1/20000 dilutionPerformed under reducing conditions.
IHC image of ab4781 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4781, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
A nuclear export sequence located on a beta-strand in fibroblast growth factor-1. - A nuclear export sequence located on a beta-strand in fibroblast growth factor-1.
Nilsen T, Rosendal KR, Sorensen V, Wesche J, Olsnes S, Wiedlocha A. J Biol Chem. 2007 Sep 7;282(36):26245-56. Epub 2007 Jul 6.
Transmembrane proteins are not required for early stages of nuclear envelope - Transmembrane proteins are not required for early stages of nuclear envelope
Ramos C, Rafikova ER, Melikov K, Chernomordik LV. Biochem J. 2006 Dec 15;400(3):393-400.