Anti-RAP1GAP antibody [Y134]

• Supplier: Abcam
• Catalog: ab32373
• Prices: $368.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: Y134
• Applications: ICC/IF ICC/IF WB IHC-P ICC/IF FC IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide corresponding to residues following the GoLoco motif of human Rap1GAP
• Blocking Peptide: RAP1GAP peptide
• Description: Rabbit Monoclonal
• Gene: RAP1GAP
• Conjugate: Unconjugated

Product images

Anti-RAP1GAP antibody [Y134] (ab32373) at 1/50000 dilution + Jurkat cell lysate

Paraffin-embedded human breast cancer tissueab32373 at 1/100 dilution

ICC/IF image of ab32373 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32373, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Overlay histogram showing SH-SY5Y cells stained with ab32373 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32373, 1/100) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Product References

Whole-genome analysis of temporal gene expression during early - Whole-genome analysis of temporal gene expression during early

Morales Johansson H, Newman DR, Sannes PL. PLoS One. 2014 Apr 1;9(4):e93413.

Podocyte-specific RAP1GAP expression contributes to focal segmental - Podocyte-specific RAP1GAP expression contributes to focal segmental

Potla U, Ni J, Vadaparampil J, Yang G, Leventhal JS, Campbell KN, Chuang PY, Morozov A, He JC, D'Agati VD, Klotman PE, Kaufman L. J Clin Invest. 2014 Apr;124(4):1757-69.

PKA and Epac synergistically inhibit smooth muscle cell proliferation. - PKA and Epac synergistically inhibit smooth muscle cell proliferation.

Hewer RC, Sala-Newby GB, Wu YJ, Newby AC, Bond M. J Mol Cell Cardiol. 2011 Jan;50(1):87-98.

Phosphorylation of Rap1GAP, a striatally enriched protein, by protein kinase A - Phosphorylation of Rap1GAP, a striatally enriched protein, by protein kinase A

McAvoy T, Zhou MM, Greengard P, Nairn AC. Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3531-6.