Anti-RAP2B antibody
| Name | Anti-RAP2B antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab58109 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Applications | WB IHC-P ICC/IF ICC/IF FC |
| Species Reactivities | Mouse, Human |
| Antigen | Recombinant full length protein, corresponding to amino acids 1-184 of Human RAP2B |
| Description | Mouse Monoclonal |
| Gene | RAP2B |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
RAP2B antibody (ab58109) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human malignant lymphoma, diffuse large B tissue.
ab58109 at 10 ug/ml staining RAP2B in human Hella cells by Immunocytochemistry/ Immunofluorescence.
![Overlay histogram showing K562 cells stained with ab58109 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58109, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in K562 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/20734_RAP2B-Primary-antibodies-ab58109-2.jpg)
Overlay histogram showing K562 cells stained with ab58109 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58109, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in K562 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.