All lanes : Anti-RASGRP3 (phospho S172) antibody [EPNCIR149] (ab166887) at 1/1000 dilution (purified)Lane 1 : LnCap wild type cell lysate transfected with RASGRP3 + Go6983Lane 2 : LnCap wild type cell lysate, untransfectedLysates/proteins at 10 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution
All lanes : Anti-RASGRP3 (phospho S172) antibody [EPNCIR149] (ab166887) at 1/1000 dilutionLane 1 : LNCap wild type cell lysate untransfected Lane 2 : LNCap wild type cell lysate transfected with RASGRP3Lane 3 : LNCap wild type cell lysate transfected with RASGRP3 + PMALane 4 : LNCap wild type cell lysate transfected with RASGRP3 + Go6983Lane 5 : LNCap wild type cell lysate transfected with RASGRP3 + Go6983 + PMALane 6 : LNCap wild type cell lysate transfected with s172A mutant RASGRP3Lane 7 : LNCap wild type cell lysate transfected with s172A mutant RASGRP3 + PMALane 8 : LNCap wild type cell lysate transfected with s172A mutant RASGRP3 + Go6983 Lane 9 : LNCap wild type cell lysate transfected with s172A mutant RASGRP3 + Go6983 + PMA Lysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution