Anti-RBCK1 antibody
| Name | Anti-RBCK1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab38540 |
| Prices | $380.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB ELISA IHC-P |
| Species Reactivities | Mouse, Human |
| Antigen | A KLH conjugated synthetic peptide (10-30 aa in length) at the N-term of first 50 aa of human RBCK1 |
| Description | Rabbit Polyclonal |
| Gene | RBCK1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-RBCK1 antibody (ab38540) at 1/100 dilution + Mouse kidney tissue lysate
Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with ab38540 at a 1/50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
ICC/IF image of ab38540 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38540, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Two coordinated mechanisms underlie tumor necrosis factor alpha-induced immediate - Two coordinated mechanisms underlie tumor necrosis factor alpha-induced immediate
Blackwell K, Zhang L, Workman LM, Ting AT, Iwai K, Habelhah H. Mol Cell Biol. 2013 May;33(10):1901-15.
Identification of RBCK1 as a novel regulator of FKBPL: implications for tumor - Identification of RBCK1 as a novel regulator of FKBPL: implications for tumor
Donley C, McClelland K, McKeen HD, Nelson L, Yakkundi A, Jithesh PV, Burrows J, McClements L, Valentine A, Prise KM, McCarthy HO, Robson T. Oncogene. 2014 Jun 26;33(26):3441-50.
Mechanism of hypoxia-induced NF-kappaB. - Mechanism of hypoxia-induced NF-kappaB.
Culver C, Sundqvist A, Mudie S, Melvin A, Xirodimas D, Rocha S. Mol Cell Biol. 2010 Oct;30(20):4901-21.