Anti-RBM14 antibody
| Name | Anti-RBM14 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab12325 |
| Prices | $370.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-P WB |
| Species Reactivities | Human, Guinea Pig, Chimpanzee, Monkey, Ape, Orangutan |
| Antigen | Immunogen was a synthetic peptide, which represented a portion of human Coactivator Activator encoded within exon 2 (LocusLink ID 10432) |
| Description | Rabbit Polyclonal |
| Gene | RBM14 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma tissue labelling RBM14 with ab12325 at 1/1000 (1µg/ml). Detection: DAB.
ICC/IF image of ab12325 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12325, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.