Anti-Rbm15 antibody
| Name | Anti-Rbm15 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab70549 |
| Prices | $370.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IP |
| Species Reactivities | Human, Rabbit, Horse, Bovine, Dog, Pig, Chimpanzee, Ferret, Monkey, Orangutan, Elephant |
| Antigen | Synthetic peptide corresponding to a region between residues 550 and 600 of human RBM15 |
| Description | Rabbit Polyclonal |
| Gene | RBM15 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunocytochemistry/Immunofluorescence analysis of HUVEC cells labelling RBM15 (red) with ab70549 at 1:100. Cells were fixed with 4% paraformaldehyde in PBS for 5 minutes, permeabilized with 0.5% Triton X-100 in PBS for 5 minutes on ice, blocked with PBS containing 10% BlockAce and incubated with the primary antibodies. An Alexa Fluor 594-conjugated anti-rabbit IgG was used as the secondary antibody. SC35 - green. RMB15 and SC35 are partially co-localized in nuclear speckles and are also present in the the nucleoplasm.
All lanes : Anti-Rbm15 antibody (ab70549) at 0.1 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µg
Detection of Human RBM15 by Western Blot of Immunprecipitate. ab70549 at 1µg/ml staining RBM15 in HeLa whole cell lysate immunoprecipitated by ab70549 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).