All lanes : Anti-RBM6 antibody [EPR15842] - C-terminal (ab188318) at 1/1000 dilutionLane 1 : HUVEC cell lysateLane 2 : A549 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-RBM6 antibody [EPR15842] - C-terminal (ab188318) at 1/5000 dilutionLane 1 : HepG2 cell lysateLane 2 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunofluorescent analysis of 4% paraformaldehyde fixed A549 cells labeling RBM6 with ab188318at 1/100 dilution. Secondary antibody Goat anti rabbit IgG (Dylight® 488) at a 1/200 dilution. Counterstained with Dapi blue.