All lanes : Anti-RBMXL2 antibody [EPR12593] (ab175228) at 1/10000 dilutionLane 1 : Mouse testis lysateLane 2 : Rat testis lysateLane 3 : Human seminoma lysateLane 4 : Jurkat lysateLane 5 : HeLa lysateLysates/proteins at 10 µg per lane.
Western blot analysis on immunoprecipitation pellet from (1) HeLa cells lysate or (2) 1X PBS (negative control) using ab175228 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Immunofluorescent analysis of Jurkat cells labeling RBMXL2 with ab175228 at 1/100 dilution.