Western Blot analysis of RCC1 in HeLa cell lysate immunoprecipitated with ab181155 at 1/50 dilution (Lane 1). Lane 2: Negative control.Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Immunofluorescent analysis of 4% paraformaldehyde-fixed A431 cells labeling RCC1 with ab181155 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 diution. Counter stained with Dapi (blue).
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling RCC1 with ab181155 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling RCC1 with ab181155 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
All lanes : Anti-RCC1 antibody [EPR5859(2)] (ab181155) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : A431 cell lysateLane 3 : Jurkat cell lysateLane 4 : 293T cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution