Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin basal cell carcinoma (left) and mouse CT26 colon carcinoma (right) tissues labelling RCC2 with ab70788 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection: DAB.
All lanes : Anti-RCC2 antibody (ab70788) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µgLane 5 : NIH3T3 whole cell lysate at 50 µg
HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) with ab70788 used for WB at 0.1 µg/ml. RCC2 was immunoprecipitated using ab70787, which recognizes an upstream epitope. Detection: Chemiluminescence with an exposure time of 1 second.