Anti-Rel B antibody [EPR7076] - C-terminal

• Supplier: Abcam
• Catalog: ab180127
• Prices: $387.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR7076
• Applications: IHC-P WB ICC/IF ICC/IF IP FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Rel B aa 550 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: RELB
• Conjugate: Unconjugated

Product images

All lanes : Anti-Rel B antibody [EPR7076] - C-terminal (ab180127) at 1/5000 dilutionLane 1 : Daudi cell lysateLane 2 : Raji cell lysateLane 3 : A549 cell lysateLane 4 : THP1 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunofluorescent analysis of A549 cells (paraformaldehyde-fixed, 4%) labeling Rel B with ab180127 at 1/100 dilution, followed by Goat anti-rabbit IgG (Dylight 555) secondary at 1/250 dilution and counter stained with DAPI.

Western blot analysis of immunoprecipitation pellet from Human Daudi lysate treated with Calyculin A and TNF-alpha immunoprecipitated using ab180127 at 1/50 dilution. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.

Flow cytometric analysis of Raji cells (paraformaldehyde-fixed, 2%) labeling Rel B with ab180127 at 1/40 dilution or a rabbit IgG (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/75 dilution.

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Rel B  with ab180127 at 1/50 dilution followed by prediluted Goat anti-rabbit IgG(HRP) secondary and counter stained with Hematoxylin.