All lanes : Anti-Relaxin 3 receptor 1 antibody (ab72914) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Brain (Human) Membrane Lysate - adult normal tissue (ab29456)Lane 3 : Spinal Cord (Human) Tissue Lysate - adult normal tissue (ab29188)Lane 4 : SK N SH (Human neuroblastoma) Whole Cell Lysate Lane 5 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.