Anti-Retinoic Acid Receptor beta antibody

• Supplier: Abcam
• Catalog: ab53161
• Prices: $391.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB ELISA IHC-P IP ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide, corresponding to amino acids 351-390 of Human Retinoic Acid Receptor beta
• Description: Rabbit Polyclonal
• Gene: RARB
• Conjugate: Unconjugated

Product images

This image shows human breast carcinoma tissue stained with ab53161 at 1/50 dilution. The left hand image shows untreated tissue; the right hand image shows tissue treated with the immunising peptide.

All lanes : Anti-Retinoic Acid Receptor beta antibody (ab53161) at 1/500 dilutionLane 1 : HepG2 cell extract, untreated.Lane 2 : HepG2 cell extract treated with the immunising peptide.

ICC/IF image of ab53161 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53161, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

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Retinoic acid signaling and the initiation of mammary gland development. - Retinoic acid signaling and the initiation of mammary gland development.

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SIRT1 suppresses beta-amyloid production by activating the alpha-secretase gene - SIRT1 suppresses beta-amyloid production by activating the alpha-secretase gene

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