Anti-RGS6 antibody (ab88086) at 1 µg/ml + rat brain tissue lysate at 50 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Anti-RGS6 antibody (ab88086) at 1 µg/ml + SJCRH30 cell lysate at 50 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
All lanes : Anti-RGS6 antibody (ab88086) at 1 µg/mlLane 1 : transfected 293T cell line lysateLane 2 : non transfected 293T cell line lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution