Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling Rho guanine exchange factor 16 with ab86068 at 1/200 (1µg/ml). Detection: DAB.
All lanes : Anti-Rho guanine exchange factor 16 antibody (ab86068) at 0.04 µg/mlLane 1 : Hela whole cell lysate at 50 µgLane 2 : Hela whole cell lysate at 15 µgLane 3 : Hela whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µgdeveloped using the ECL technique
Detection of Rho guanine exchange factor 16 by Western blot of Immunprecipitate. ab86068, at 1 µg/ml, staining Rho guanine exchange factor 16 in HeLa whole cell lysate immunoprecipitated using ab86068 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 3 seconds.