Anti-RHOJ antibody
| Name | Anti-RHOJ antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab57584 |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | WB ICC/IF ICC/IF FC |
| Species Reactivities | Human |
| Antigen | Recombinant full length protein, corresponding to amino acids 1-215 of Human RHOJ |
| Description | Mouse Monoclonal |
| Gene | RHOJ |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HepG2 cells stained with ab57584 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57584, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/22984_ab57584-3-ab57584FC.jpg)
Overlay histogram showing HepG2 cells stained with ab57584 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57584, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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Product References
RhoJ interacts with the GIT-PIX complex and regulates focal adhesion disassembly. - RhoJ interacts with the GIT-PIX complex and regulates focal adhesion disassembly.
Wilson E, Leszczynska K, Poulter NS, Edelmann F, Salisbury VA, Noy PJ, Bacon A, Rappoport JZ, Heath JK, Bicknell R, Heath VL. J Cell Sci. 2014 Jul 15;127(Pt 14):3039-51.
RhoJ/TCL regulates endothelial motility and tube formation and modulates - RhoJ/TCL regulates endothelial motility and tube formation and modulates
Kaur S, Leszczynska K, Abraham S, Scarcia M, Hiltbrunner S, Marshall CJ, Mavria G, Bicknell R, Heath VL. Arterioscler Thromb Vasc Biol. 2011 Mar;31(3):657-64. doi:
Coordinated RhoA signaling at the leading edge and uropod is required for T cell - Coordinated RhoA signaling at the leading edge and uropod is required for T cell
Heasman SJ, Carlin LM, Cox S, Ng T, Ridley AJ. J Cell Biol. 2010 Aug 23;190(4):553-63.