Anti-RIP140 antibody - ChIP Grade

• Supplier: Abcam
• Catalog: ab42126
• Prices: $387.00
• Sizes: 50 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-P IHC-P IHC-F IP WB ICC/IF ICC/IF ChIP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (Human) corresponding to the C terminal domain of RIP140
• Description: Rabbit Polyclonal
• Gene: NRIP1
• Conjugate: Unconjugated

Product images

ab42126 (4µg/ml) staining RIP140 in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the nuclei of the intestinal glands.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

ICC/IF image of ab42126 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42126, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

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Wu CY, Feng X, Wei LN. Nucleic Acids Res. 2014 Apr;42(7):4306-17.

NRIP1/RIP140 siRNA-mediated attenuation counteracts mitochondrial dysfunction in - NRIP1/RIP140 siRNA-mediated attenuation counteracts mitochondrial dysfunction in

Izzo A, Manco R, Bonfiglio F, Cali G, De Cristofaro T, Patergnani S, Cicatiello R, Scrima R, Zannini M, Pinton P, Conti A, Nitsch L. Hum Mol Genet. 2014 Aug 15;23(16):4406-19.

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Lapierre M, Bonnet S, Bascoul-Mollevi C, Ait-Arsa I, Jalaguier S, Del Rio M, Plateroti M, Roepman P, Ychou M, Pannequin J, Hollande F, Parker M, Cavailles V. J Clin Invest. 2014 May;124(5):1899-913.

NF-kappaB-mediated degradation of the coactivator RIP140 regulates inflammatory - NF-kappaB-mediated degradation of the coactivator RIP140 regulates inflammatory

Ho PC, Tsui YC, Feng X, Greaves DR, Wei LN. Nat Immunol. 2012 Mar 4;13(4):379-86.

Cognitive impairments in adult mice with constitutive inactivation of RIP140 gene - Cognitive impairments in adult mice with constitutive inactivation of RIP140 gene

Duclot F, Lapierre M, Fritsch S, White R, Parker MG, Maurice T, Cavailles V. Genes Brain Behav. 2012 Feb;11(1):69-78.

Promyelocytic leukemia protein in retinoic acid-induced chromatin remodeling of - Promyelocytic leukemia protein in retinoic acid-induced chromatin remodeling of

Chuang YS, Huang WH, Park SW, Persaud SD, Hung CH, Ho PC, Wei LN. Stem Cells. 2011 Apr;29(4):660-9.

Chronic AMPK activation evokes the slow, oxidative myogenic program and triggers - Chronic AMPK activation evokes the slow, oxidative myogenic program and triggers

Ljubicic V, Miura P, Burt M, Boudreault L, Khogali S, Lunde JA, Renaud JM, Jasmin BJ. Hum Mol Genet. 2011 Sep 1;20(17):3478-93.

Genetic variations strongly influence phenotypic outcome in the mouse retina. - Genetic variations strongly influence phenotypic outcome in the mouse retina.

Jelcick AS, Yuan Y, Leehy BD, Cox LC, Silveira AC, Qiu F, Schenk S, Sachs AJ, Morrison MA, Nystuen AM, DeAngelis MM, Haider NB. PLoS One. 2011;6(7):e21858.

Perilipin overexpression in white adipose tissue induces a brown fat-like - Perilipin overexpression in white adipose tissue induces a brown fat-like

Sawada T, Miyoshi H, Shimada K, Suzuki A, Okamatsu-Ogura Y, Perfield JW 2nd, Kondo T, Nagai S, Shimizu C, Yoshioka N, Greenberg AS, Kimura K, Koike T. PLoS One. 2010 Nov 16;5(11):e14006.

Activation of testicular orphan receptor 4 by fatty acids. - Activation of testicular orphan receptor 4 by fatty acids.

Tsai NP, Huq M, Gupta P, Yamamoto K, Kagechika H, Wei LN. Biochim Biophys Acta. 2009 Nov-Dec;1789(11-12):734-40. doi: