All lanes : Anti-RIT2 antibody [1G2] (ab119082) at 1/2000 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY control Lane 2 : HEK293T cells transfected with pCMV6-ENTRY RIT2Lysates/proteins at 5 µg per lane.
Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RIT2 using ab119082 at a dilution of 1/100.
HEK293T cells transfected with either RC205367 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ab119082 at a dilution of 1/100, and then analyzed by flow cytometry.