Anti-RNA Helicase A antibody
| Name | Anti-RNA Helicase A antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab54593 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | IP WB IHC-P FC ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Recombinant fragment: MGDVKNFLYA WCGKRKMTPT YEIRAVGNKN RQKFMCEVQV EGYNYTGMGN STNKKDAQSN AARDFVNYLV RINEIKSEEV PAFGVASPPP , corresponding to amino acids 1-91 of Human RNA Helicase A Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | DHX9 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
RNA Helicase A antibody (ab54593) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human testis.
ab54593 at 10 µg/ml staining RNA Helicase A in human Hela cells by Immunocytochemistry / Immunofluorescence.
![Overlay histogram showing HeLa cells stained with ab54593 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54593, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/23509_RNA-Helicase-A-Primary-antibodies-ab54593-2.jpg)
Overlay histogram showing HeLa cells stained with ab54593 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54593, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
Host factors that interact with the pestivirus N-terminal protease, Npro, are - Host factors that interact with the pestivirus N-terminal protease, Npro, are
Jefferson M, Donaszi-Ivanov A, Pollen S, Dalmay T, Saalbach G, Powell PP. J Virol. 2014 Sep;88(18):10340-53.
Identification of RNA helicase A as a cellular factor that interacts with - Identification of RNA helicase A as a cellular factor that interacts with
Lin L, Li Y, Pyo HM, Lu X, Raman SN, Liu Q, Brown EG, Zhou Y. J Virol. 2012 Feb;86(4):1942-54.
A functional nuclear epidermal growth factor receptor, SRC and Stat3 heteromeric - A functional nuclear epidermal growth factor receptor, SRC and Stat3 heteromeric
Jaganathan S, Yue P, Paladino DC, Bogdanovic J, Huo Q, Turkson J. PLoS One. 2011 May 5;6(5):e19605.
Evidence that Lin28 stimulates translation by recruiting RNA helicase A to - Evidence that Lin28 stimulates translation by recruiting RNA helicase A to
Jin J, Jing W, Lei XX, Feng C, Peng S, Boris-Lawrie K, Huang Y. Nucleic Acids Res. 2011 May;39(9):3724-34.
Enhanced gene silencing in cells cured of persistent virus infection by RNA - Enhanced gene silencing in cells cured of persistent virus infection by RNA
Pelletier I, Saulnier A, Brisac C, Jegouic S, Vabret N, Tangy F, Blondel B, Colbere-Garapin F. J Virol. 2010 Jul;84(13):6880-5.
Lin28-mediated post-transcriptional regulation of Oct4 expression in human - Lin28-mediated post-transcriptional regulation of Oct4 expression in human
Qiu C, Ma Y, Wang J, Peng S, Huang Y. Nucleic Acids Res. 2010 Mar;38(4):1240-8.
An antiviral response directed by PKR phosphorylation of the RNA helicase A. - An antiviral response directed by PKR phosphorylation of the RNA helicase A.
Sadler AJ, Latchoumanin O, Hawkes D, Mak J, Williams BR. PLoS Pathog. 2009 Feb;5(2):e1000311.