All lanes : Anti-RNF11 antibody (ab171385) at 1/100 dilutionLane 1 : Non-transfected 293 cell lysateLane 2 : RNF11 transfected 293 cell lysateLysates/proteins at 2 µg per lane.
Immunohistochemistry analysis in formalin fixed and paraffin embedded Human breast carcinoma labeling RNF11 with ab171385 at 1/10 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram) labeling RNF11 with ab171385 at 1/10 dilution. FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.