Anti-RNF144 antibody
| Name | Anti-RNF144 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab75054 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ICC/IF ICC/IF WB ELISA |
| Species Reactivities | Human, Monkey, Mouse |
| Antigen | Synthetic peptide derived from an internal sequence of human RNF144 |
| Description | Rabbit Polyclonal |
| Gene | RNF144A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of RNF144 staining in human prostate formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab75054, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ICC/IF image of ab75054 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab75054 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-RNF144 antibody (ab75054) at 1/500 dilutionLane 1 : extracts from COLO cellsLane 2 : extracts from COLO cells with immunising peptide at 5 µgLysates/proteins at 5 µg per lane.
Product References
RNF144A, an E3 ubiquitin ligase for DNA-PKcs, promotes apoptosis during DNA - RNF144A, an E3 ubiquitin ligase for DNA-PKcs, promotes apoptosis during DNA
Ho SR, Mahanic CS, Lee YJ, Lin WC. Proc Natl Acad Sci U S A. 2014 Jul 1;111(26):E2646-55. doi:
Metastasis-associated protein 1 drives tumor cell migration and invasion through - Metastasis-associated protein 1 drives tumor cell migration and invasion through
Marzook H, Li DQ, Nair VS, Mudvari P, Reddy SD, Pakala SB, Santhoshkumar TR, Pillai MR, Kumar R. J Biol Chem. 2012 Feb 17;287(8):5615-26.